Mengenal pasti tahap pengetahuan pelajar tahun akhir Ijazah Sarjana Muda Kejuruteraan di KUiTTHO dalam bidang keusahawanan dari aspek pengurusan modal

Malaysia ialah sebuah negara membangun di dunia. Dalam proses pembangunan ini, hasrat negara untuk melahirkan bakal usahawan beijaya tidak boleh dipandang ringan. Oleh itu, pengetahuan dalam bidang keusahawanan perlu diberi perhatian dengan sewajarnya; antara aspek utama dalam keusahawanan ialah modal. Pengurusan modal yang tidak cekap menjadi punca utama kegagalan usahawan. Menyedari hakikat ini, kajian berkaitan Pengurusan Modal dijalankan ke atas 100 orang pelajar Tahun Akhir Kejuruteraan di KUiTTHO. Sampel ini dipilih kerana pelajar-pelajar ini akan menempuhi alam pekeijaan di mana mereka boleh memilih keusahawanan sebagai satu keijaya. Walau pun mereka bukanlah pelajar dari jurusan perniagaan, namun mereka mempunyai kemahiran dalam mereka cipta produk yang boleh dikomersialkan. Hasil dapatan kajian membuktikan bahawa pelajar-pelajar ini berminat dalam bidang keusahawanan namun masih kurang pengetahuan tentang pengurusan modal terutamanya dalam menentukan modal permulaan, pengurusan modal keija dan caracara menentukan pembiayaan kewangan menggunakan kaedah jualan harian. Oleh itu, satu garis panduan Pengurusan Modal dibina untuk memberi pendedahan kepada mereka

Maze warriors

Light travels in a straight line and never curves, but changes its direction through reflection. The light ray that strikes a surface is called the incident ray, while the one that bounces off is the reflected ray. All reflected light obeys the Snell’s Law, that the angle of incidence equals the angle of reflection

Effect of ethanol treatment on shrinkage of oil palm trunk for the drying process

Oil palm trunk is one of the promising biomass materials due to the high volume of unused waste components and increasing worldwide demand to replace conventional wood. This study aimed to investigate the feasibility of using ethanol as a drying agent for oil palm trunks with different dimensional surfaces (radial, tangential and longitudinal sections). Radial shrinkage percentage for the outer layer is 1.50% (untreated) and 1.22 % (treated) respectively. In comparison, the inner layer of the untreated sample was recorded at 2.54 % shrinkage and the treated sample was at 2.29%. The tangential sample for the inner untreated sample shows 2.60% and the treated sample shows 2.40%. The same pattern of shrinkage was shown for the tangential section on the outer layer as 1.81% and 1.10% of the untreated and treated sample respectively. For the longitudinal surface, the inner layer section of the untreated sample was recorded at 0.39% compared to the treated sample at 0.25%. In comparison, a longitudinal surface section for the outer layer of the untreated sample was recorded at 0.38% while the treated sample was recorded at 0.33% shrinkage percentage. The effect of ethanol treatment on the shrinkage is significantly different between different sections (P-value: 0.01) and between the outer and inner layers (P-value: 0.02). The result suggested that ethanol treatment could be an option for the oil palm trunk drying process. Dried oil palm trunk can be utilized as a potential substitution of biomass and wood to produce various products

Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies

The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canine synovial fluid for proteome studies. Pooled synovial fluid samples were obtained from clinically healthy dogs (n=5), with no radiographic features of osteoarthritis. The acetone precipitation method and 10% w/v of trichloroacetic acid/acetone were chosen to deplete the albumin from canine synovial fluid and the outcome from the different methods were compared using one dimensional and two-dimensional gel electrophoresis studies. The results showed that the 10% w/v TCA/acetone precipitation method removed highly abundant proteins from synovial fluid for gel electrophoresis studies compared to the acetone precipitation method. The 10% w/v TCA/acetone precipitation method provides an effective method to remove albumin from the synovial fluid using gel electrophoresis, especially two-dimensional gel electrophoresis. The accessibility and cost of TCA and acetone make this method of precipitation a simple and cost-effective technique in preparing a canine synovial fluid for two-dimensional gel electrophoresis analysis

Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models

Background: Osteoarthritis (OA) is a multifaceted condition that affects both the subchondral bones and the articular cartilage. Animal models are widely used as an effective supplement and simulation for human OA studies in investigating disease mechanisms and pathophysiology. This study is aimed to evaluate the temporal changes of bone and cartilage in surgically and chemically induced osteoarthritis using micro-computed tomography and histology. Methods: Thirty rabbits underwent either anterior cruciate ligament transection (ACLT) procedure or injected intraarticularly with monosodium iodoacetate (MIA, 8 mg) at the right knee joint. The subchondral bones were scanned via micro-CT, and articular cartilage was assessed histologically at 4-, 8- and 12-week post-induction. Results: Based on bone micro-architecture parameters, the surgically induced group revealed bone remodelling processes, indicated by increase bone volume, thickening of trabeculae, reduced trabecular separation and reduced porosity. On the other hand, the chemically induced group showed active bone resorption processes depicted by decrease bone volume, thinning of trabeculae, increased separation of trabecular and increased porosity consistently until week 12. Histologically, the chemically induced group showed more severe articular cartilage damage compared to the surgically induced group. Conclusions: It can be concluded that in the ACLT group, subchondral bone remodelling precedes articular cartilage damage and vice versa in the MIA group. The findings revealed distinct pathogenic pathways for both induction methods, providing insight into tailored therapeutic strategies, as well as disease progression and treatment outcomes monitoring

Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits

Background: Animal models are significant for understanding human osteoarthritis (OA). This study compared the synovial fluid proteomics changes in surgical and chemical induced OA models. Methods: Thirty rabbits either had anterior cruciate ligament transection (ACLT) procedure or injected intra-articularly with monosodium iodoacetate (MIA, 8 mg) into the right knee. The joints were anatomically assessed, and the synovial fluid proteins analyzed using two-dimensional polyacrylamide gel electrophoresis (2DGE) and MALDI TOF/TOF mass spectrometry analysis at 4, 8 and 12 weeks. The proteins' upregulation and downregulation were compared with control healthy knees. Results: Seven proteins (histidine-rich glycoprotein, beta-actin-like protein 2 isoform X1, retinol-binding protein-4, alpha-1-antiproteinase, gelsolin isoform, serotransferrin, immunoglobulin kappa-b4 chain-C-region) were significantly expressed by the surgical induction. They characterized cellular process (27%), organization of cellular components or biogenesis (27%), localization (27%) and biological regulation (18%), which related to synovitis, increased cellularity, and subsequently cartilage damage. Three proteins (apolipoprotein I-IV precursor, serpin peptidase inhibitor and haptoglobin precursor) were significantly modified by the chemical induction. They characterized stimulus responses (23%), immune responses (15%), biological regulations (15%), metabolism (15%), organization of cellular components or biogenesis (8%), cellular process (8%), biological adhesions (8%) and localization (8%), which related to chondrocytes glycolysis/death, neovascularization, subchondral bone necrosis/collapse and inflammation. Conclusions: The surgical induced OA model showed a wider range of protein changes, which were most upregulated at week 12. The biological process proteins expressions showed the chemical induced joints had slower OA progression compared to surgical induced joints. The chemical induced OA joints showed early inflammatory changes, which later decreased

Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies

The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canine synovial fluid for proteome studies. Pooled synovial fluid samples were obtained from clinically healthy dogs (n=5), with no radiographic features of osteoarthritis. The acetone precipitation method and 10% w/v of trichloroacetic acid/acetone were chosen to deplete the albumin from canine synovial fluid and the outcome from the different methods were compared using one dimensional and twodimensional gel electrophoresis studies. The results showed that the 10% w/v TCA/acetone precipitation method removed highly abundant proteins from synovial fluid for gel electrophoresis studies compared to the acetone precipitation method. The 10% w/v TCA/acetone precipitation method provides an effective method to remove albumin from the synovial fluid using gel electrophoresis, especially two-dimensional gel electrophoresis. The accessibility and cost of TCA and acetone make this method of precipitation a simple and cost-effective technique in preparing a canine synovial fluid for two-dimensional gel electrophoresis analysis